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Downloaded from: www.freefilegate.com on October 22, 2020 by guest. [20] pdf. ( The material on. C Test Your Aptitude-Venugopal 2006-10-01. Candidate Will Download C Test. Author: Vedav Pradeep Author: Venugopal K R Venugopal. How Is The CThe long term objectives of this proposal are to determine the mechanism of regulation of the gene encoding proinsulin (PI) in a cell line that is capable of expressing multiple copies of the PI gene, and to identify the essential cis-regulatory elements of the PI gene. Previous studies have demonstrated that the tissue-specific expression of the PI gene is regulated by a negative cis-acting element located within 500 bp of the PI promoter, which is termed the PI enhancer. The PI enhancer is inactive in many cell types, but active in insulin producing cells, whereas the PI gene is inactive in these cell types. Thus, the PI enhancer functions as a tissue-specific negative cis-regulatory element. Current evidence indicates that the PI enhancer contains a complex promoter, consisting of 4 cis-elements (W, NG, N, and B). The in vivo activity of these elements is characterized by using PI enhancer-reporter constructs. In addition, the cis-elements contained in the PI enhancer are mutagenized, and the effects of these mutations on the in vivo activity of the PI enhancer are determined. These studies have identified a cis-acting element in the W element, which is essential for the activity of the W element. In order to test whether the W element itself functions as a tissue-specific negative cis-regulatory element, the structure of the W element is analyzed 0b46394aab
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